SpectraMax ® M2 Multi-Mode Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. AlphaScreen. SpectraMax Mini User Guide. All . 1. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 5 12. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Contact our support team with your questions regarding Molecular Devices products and applications. Fully tested with validations, warranty. . Table 1. The plate reader can measure multiple readouts, including UV and visible absorbance. Other technologies such as imaging. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Highly customizable low volume. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. It was also read on the monochromator-based. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. The Gemini EM can sample a variety of formats, from 6- to 384-well. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Positions H21-K22 contained plate blanks of PBS only. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. 7°C (gradient heating design). The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. Instrument settings used with the SpectraMax M5 Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. A. ELISA Plate Readers / Microplate Readers. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Endpoint, kinetic, spectrum. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 20 μL of Component A was added to each well of the microplate. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The advanced optical and. It also integrates with the. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Temperature control. Step 4: Incubate at room temperature for 1 hour, protected from light. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The calculated LOQ values were 0. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. We would like to show you a description here but the site won’t allow us. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. 0 out of 5 $ 5,999. If it looks fine, then proceed. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Wide range of applications. Microplate Readers Biotek Epoch Microplate Reader. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Most papers they cultured bacteria to OD600>0. The screw remains inside the instrument. Cell Cytotoxicity assay procedure. Example: [Drawer]. 100 fmol 384-well (75 µL) 0. Absorbance (optical density, OD) was read at 570 nm and 605. Add to Cart. Products : General, Microplate Readers. Sensitive fluorescent quantitation of DNA with the Quant-iT. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. Download the SpectraMax Mini Reader Plug-in and associated guides. Data exemplifying linear ranges from the three different kits are presented in Figure 2. Microplate readers’ standard detection modes. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Unlock your i3/i3x instrument after shipment. Prerequisites: SoftMax Pro 7. The drawing shows a standard microplate of 15 mm height covered with a lid. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. \ Figure 3. Molecular Devices: Molecular Devices. Use the provided 3. Cell Analysis. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 0 out of 5 $ 5,999. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. 7°C (gradient heating design). The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. FI assay. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 1. Introduction. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. limit of detection (LLD) for ATP was estimated for each microplate reader using the . It features a modular design that allows users to add or change detection cartridges according to their needs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. microplate reader. Temperature control. Molecular Devices SpectraMax M2E Microplate Reader. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. M2. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Download PDF versions of the SpectraMax Microplate Reader User Guides. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. View the Molecular Devices website. 6% for the on-board optics (monochromators). following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SoftMax® Pro 7. The SpectraMax® iD3 Multi Mode Microplate . 303 unable to cal dark current. 1. View the Molecular Devices website. both cuvette-based and microplate reader applications on the same instrument. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Multimode Microplate Reader. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. 1. Contents of wells were pipetted up and down several times to mix. 316 grating motor driver faulty. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Figure 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. The 2-channel configuration is available with 0, 2, or 4 injectors. Cell Cytotoxicity assay procedure. Or, with the reader specifications noted from the Instrument Description section of your reader's. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Molecular Devices SpectraMax M2 Multimode Plate Reader. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Also see:. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. . 0 out of 5 $ 11,999. In addition, the SpectraMax® iD5 can be expanded to include bottom. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 380 nm/Exc. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The CellTiter-Glo assay from Promega uses luciferase. The screw remains inside the instrument. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. SpectraMax M5 reader settings are shown. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Table 1. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. 305 reference level saturation. 76358-624. ATP standard curves read on SpectraMax microplate readers. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Type:Spectrophotometer. Category 2 and 3 microplate readers use gradient heating as well. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Other features and benefits include: Eliminate changing out filters. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. SpectraMax® Microplate Readers and SoftMax® Pro Software. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 0 out of 5 $ 9,995 $ 9,495. 030 fmol Fluorescence Polarization (FP). What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. Table of Contents. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Detailed specifications of each model reader are reported in Section. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Five preferred detection modes. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 5 12. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 7°C and the bottom to 36. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. Users can perform spectral scans from 250 nm to 750. See SpectraMax® Microplate Reader User Manual Download Page. Microplate reader assays requiring infrared (IR) or red-shifted. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Compact designs. 5 87. These robust readers have been placed in labs from. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Microplate Automation & Detection. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. The drawing shows a standard microplate of 15 mm height covered with a lid. Page 28 Chapter 5: Read Modes and Read Types 1. Use the provided 3. We would like to show you a description here but the site won’t allow us. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. SpectraMax iD5 Multi-Mode Microplate Reader. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 100 fmol 1536-well (8 µL) 0. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. In addition, the SpectraMax® iD5 can be expanded to include bottom. 0 out of 5 $ 6,499. Molecular Devices Spectramax iD3 Microplate Reader. Note that this procedure is only for the SpectraMax L model. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. For readers without a cuvette port, these options are not shown in the software. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. For a higher . The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The SpectraMax® ABS Plus Microplate Reader can run . Omnia® Compatible Microplate Reader Documentation Version No. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. In addition, the iD5 reader measures TRF and FP. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Example: [Drawer]. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. The following document is intended to demonstrate setup of this instrument. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. 6% for the on-board optics (monochromators). Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. In . 304 signal level saturation. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). Fluorescence Microplate Readers. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. HTRF-compatible SpectraMax Multi-Mode Readers. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). These lab workhorses are used in drug discovery, research, bioassay. 305 reference level saturation. The SpectraMax® ABS Plus Microplate Reader can run . Total volume (except deep. On the back, in the right hand top corner,. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. 2 only) SpectraMax M-series Microplate readers. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Align the two slots on the bottle holder with the two pegs on the rail. . With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. Microplate reader settings. 315 can't find zero order. Click SoftMax Pro Software User Guide. The calculated LOQ values were 0. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Find regional support. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. zip. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. 7°C and the bottom to 36. Measuring platelet aggregation with a microplate reader. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Microplate Readers BioTek Epoch 2 Microplate Reader. San Jose, Calif. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection.